|
KMID : 0613820190290101126
|
|
Journal of Life Science
2019 Volume.29 No. 10 p.1126 ~ p.1135
|
|
|
Isolation of the Protease-producing Yeast Pichia anomala CO-1 and Characterization of Its Extracellular Neutral Protease
|
|
Kim Ji-Yeon
|
|
|
Abstract
|
|
|
|
From a sample of bamboo byproduct, the protease-producing yeast strain CO-1 was newly isolated. Strain CO-1 is spherical to ovoid in shape and measures 3.1?4.0×3.8?4.4 μm. For the growth of strain CO-1, the optimal temperature and initial pH were 30°C and 4.0, respectively. The strain was able to grow in 0.0?15.0%(w/v) NaCl and 0.0-9.0%(v/v) ethanol. Based on a phylogenetic analysis of its 18S rDNA sequences, strain CO-1 was identified as Pichia anomala. The extracellular protease produced by P. anomala CO-1 was partially purified by ammonium sulfate precipitation, which resulted in a 14.6-fold purification and a yield of 7.2%. The molecular mass of the protease was recorded as approximately 30 kDa via zymogram. The protease activity reached its maximum when 1.0%(w/v) CMC was used as the carbon source, 1.0%(w/v) yeast extract was used as the nitrogen source, and 0.3%(w/v) MnSO4 was used as the mineral source. The protease revealed the highest activity at pH 7.0 and 30°C. This enzyme maintained more than 75% of its stability at a pH range of 4.0-10.0. After heating at 65℃ for 1 hr, the neutral protease registered at 60% of its original activity. The protease production coincided with growth and attained a maximal level during the post-exponential phase.
|
|
|
KEYWORD
|
|
|
Characterization, extracellular neutral protease, identification, optimization, Pichia anomala
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|